Microwave oven-based technique for immunofluorescent staining of paraffin-embedded tissues
نویسندگان
چکیده
منابع مشابه
A new technique for Gram staining paraffin-embedded tissue.
Five techniques for Gram staining bacteria in paraffin sections were compared on serial sections of pulmonary tissues from eight bacteriological necropsies. Brown and Hopp's method was the most satisfactory for distinguishing Gram-positive and Gram-negative bacteria. However, this method cannot be recommended as the preparations were frequently overstained, and the Gram-negative bacteria were s...
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The main problems in identifying Treponema pallidum in tissues are optical definition contrast, and specificity. In general, fluorochrome staining provides optical definition and contrast superior to that obtained by ordinary tinctorial staining, and in theory improved resolution. Specificity is lacking however, as with other stains. In contrast, immunofluorescence should combine the optical ad...
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The fluorescence in situ hybridization (FISH) technique is a sensitive method for detecting gene amplification in tumor cells. However, FISH on formalin-fixed and paraffin-embedded tissue sections is time-consuming and labor-intensive (1). Tissue processing and cross-linking fixatives such as formalin may prevent the probe from accessing its target sequence, prolonging the FISH processing time ...
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introduction: htlv-1 , the first known human retrovirus belongs to oncovirus subfamily of retroviruses. the major characteristic of htlv-1 is its highly restricted geographic prevalence. northern part of khorasan is an endemic region of htlv-1 infection. epidemiological studies can help in designing preventive programs for htlv-1 infection. the aim of this study was the establishment of a pcr t...
متن کاملAn enhanced antigen-retrieval protocol for immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.
Formalin is the most commonly used fixative for light microscopy because of its preservation of -morphological details. A major adverse effect of formalin fixation is formation of cross-linkages between epitopes (amino acid residues) and unrelated proteins by formaldehyde groups. The great majority of monoclonal and polyclonal antibodies used for immunohistochemical (IHC) staining of formalin-f...
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ژورنال
عنوان ژورنال: Journal of Molecular Histology
سال: 2007
ISSN: 1567-2379,1567-2387
DOI: 10.1007/s10735-007-9093-6